What do these products do in this instrument?
Syringe pumps: multiple-reagent aspiration and dispensing
Organize aspiration and dispensing in syringe-driven reagent branches, selecting the actual source through valve position. Confirm standby, mixing and replenishment conditions separately for bead suspensions, binding reagents and substrates.
HLD and HMD organize aspiration/dispensing paths with rotary and solenoid valves respectively. Capacity budgets include useful volume, holding stages and replenishment points; valve states and pump actions together create each delivery.
Organize syringe metering around aspiration, holding and dispensing
Syringe drives use controlled displacement for aspiration, holding and dispensing. With direct contact, source liquid enters the metering chamber; with system-liquid drive, sample can remain at the probe tip or a designated tube segment. Define wetted coverage before capacity, cleaning and replenishment. For repeated dispensing, track current inventory, delivered volume and next replenishment time.
Further technical reading
Map valve connections to every action
Check port connections, opening/closing sequence and power-off state separately for rotary- and solenoid-valve configurations. Changing source does not automatically remove old liquid from the chamber or shared tubing. Plan flushing for formulation and residue requirements, then evaluate the first dose after switching and dose-to-dose variation.
Translate actual branch conditions into configuration requirements
| Condition | To confirm |
|---|---|
| Stored volume and timing | Define volume per operation, consecutive dispensing count, holding time and permitted replenishment window. |
| Valve positions and destinations | Provide a source, receiver and waste port diagram with open/closed and power-off states. |
| Liquid changes and first dose | Check shared residue, priming destination and actual received volume after standby or source changes. |
| Operation | Specific operating conditions |
|---|---|
| Multiple-reagent aspiration and dispensing | Dose per operation, total cycle volume, replenishment/bottle-change interval, backpressure and receiving geometry |
Compare product configurations by the roles on this page
| Product | Configuration option | Conditions to verify |
|---|---|---|
| HLD rotary-valve syringe pump | Aspiration, holding and dispensing with a rotary valve | Check source/outlet valve positions, capacity budget and replenishment timing |
| HMD solenoid-valve syringe pump | Metered aspiration and dispensing with solenoid valves | Check open/closed and power-off states, working volume and replenishment cycle |
Explore specific operating and validation tasks
Multiple-reagent aspiration and dispensing
Bead suspensions, binding reagents and substrates may have different settling, light and storage requirements. Plan standby, mixing and first-dose recovery by actual formulation, including residual liquid in shared tubing.
Confirm product roles in actual operation
| Operation | Measurements and observations | Priority checks |
|---|---|---|
| Multiple-reagent aspiration and dispensing | Use actual media to check first, middle and last doses and delivery after bottle changes; confirm received volume and timing rather than substituting commanded steps for volume | Empty sources, bubbles, nozzle droplets and backpressure changes affect first or last doses |
Evaluate reagent delivery, post-wash residue, bead loss and assay background together.
For repeated dispensing from one chamber, is checking total volume enough?
Measure each received dose, especially the first and last. Even when total volume is near target, individual errors may differ. Relate replenishment, valve switching and receiving actions to each result.
Pump flow alone does not prove adequate washing; validate magnetic capture, mixing and aspiration position across the whole station.