Locate the source, drive and receiving position for this task
Define actual sample, diluent or internal-standard volumes, concentration at each stage, mixing and subsequent sampling conditions. Follow the diagram to confirm liquid contact, pressure and destination at each position before assigning drive and connection roles.
Calculate cycle volume from dilution ratio and well count
Calculate sample, diluent and total cycle volume from the ratio and well count, including first and last doses and retained volume. Small sample aliquots may need a different tip or program. Adequate capacity does not establish acceptable ratio error.
| Condition to compare | Information required | Selection affected |
|---|---|---|
| Dose ratios | Minimum original-sample volume and dilution volumes at each level | Compare actual delivery with the capacity range |
| Well layout | Well count, sequence, mixing and tip changes | Prevent identity and ratio mismatches across dilution stages |
| Mixing conditions | Usable vessel volume and mixing depth | Check consistency of subsequent sampling |
Compare product options against task requirements
The following candidate options match this page's responsibilities. Compare them against the operating conditions described, then check capacity, materials and interfaces on the product pages. Card images show representative configurations; suitability must still be confirmed at actual operating points and by application results.
| Product category | Corresponding role | Confirm before selection |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Preserve identity through mixing, tip changes and sampling at each level
Perform sampling, diluent addition and mixing in sequence, changing tips according to contamination controls. Record each source in serial dilution. Preserve completed-well records after replenishment or faults to prevent duplicate dosing.
Action records should link commands, valve or interface states and actual received results. When raw material, container or sample identity changes, reconfirm shared paths and conditions permitting the next step. Completion of drive movement alone does not replace task acceptance.
Include minimum sample aliquots and first and last wells
Measure sample and diluent delivery first, then validate mixture ratio and method results. Include first and last wells, minimum sample aliquots, replenishment and different liquid classes.
| Validation scenario | Measurement or observation | Interpretation and action |
|---|---|---|
| Minimum sample volume | Measure delivered volumes and mixed ratio separately | Check tip and air-column condition when small-volume delivery fails |
| First and last wells and serial stages | Cover first and last wells and subsequent sampling | Distinguish dose, mixing and propagated stage-to-stage errors |
Record test medium, configuration, installed path and operating sequence; present routine operating points and boundary results separately. Calculations support design discussions, component documentation checks models, and installed measurements confirm the actual task.
After an abnormality or maintenance, first identify which segment needs recovery
Small-volume error, shared-path residue or insufficient mixing can change the actual ratio. Check results from the affected step before resuming the next action.
Retain current source, container identity, completed actions, valve or interface state and actual receiving information. Use the table to locate the stage that changed before deciding to retry, continue or restart. After recovery, recheck the relevant operating point before returning an uncertain action to the automatic cycle.