Locate the source, drive and receiving position for this task
Define actual sample, diluent or internal-standard volumes, concentration at each stage, mixing and subsequent sampling conditions. Follow the diagram to confirm liquid contact, pressure and destination at each position before assigning drive and connection roles.
Convert concentration targets into preparation volumes at each stage
Calculate preparation volumes from the target concentration. Capacity must accommodate both the small stock-solution volume and the larger diluent volume. For serial preparation, record volume, mixing and sampling at every stage rather than only the final dilution factor.
| Condition to compare | Information required | Selection affected |
|---|---|---|
| Volumes at each level | Starting concentration, target concentration and total volume at each level | Compare independent preparation with serial dilution |
| Matrix changeover | Contact paths for standards, carriers and blanks | Control shared-section residue |
| Secondary sampling | Available mixed-solution volume and sampling volume | Check minimum working volume and holding time |
Compare product options against task requirements
The following candidate options match this page's responsibilities. Compare them against the operating conditions described, then check capacity, materials and interfaces on the product pages. Card images show representative configurations; suitability must still be confirmed at actual operating points and by application results.
| Product category | Corresponding role | Confirm before selection |
|---|---|---|
| HLD rotary-valve syringe pump | Aspiration, holding and dispensing with a rotary valve | Check source/outlet valve positions, capacity budget and replenishment timing |
| MRV3 multiport rotary valve | Multiple-source selection | Compare 10-, 16- and 24-port options and port diagrams; verify high-pressure suitability separately |
Change matrices while preserving diluted-sample identity
Confirm sources and blanks before metering and mixing. Clear shared sections after changing concentration or matrix. When repeating an over-range sample, identify the source sample and original-cycle result to prevent mismatches.
Action records should link commands, valve or interface states and actual received results. When raw material, container or sample identity changes, reconfirm shared paths and conditions permitting the next step. Completion of drive movement alone does not replace task acceptance.
Validate using ratios, blanks and method response
Measure delivery from both paths, actual ratio and method response, covering the most challenging concentration and matrix combinations. Standard results validate preparation and mixing; they do not guarantee arbitrarily small pump doses.
| Validation scenario | Measurement or observation | Interpretation and action |
|---|---|---|
| Lowest concentration level | Measure ratio, uniformity and appropriate blanks | Check small-dose errors, adsorption and propagation of preceding-stage errors |
| Recovery after standard changeover | Switch from high to low concentration and record results | Use signal recovery to define flushing and washing procedures |
Record test medium, configuration, installed path and operating sequence; present routine operating points and boundary results separately. Calculations support design discussions, component documentation checks models, and installed measurements confirm the actual task.
After an abnormality or maintenance, first identify which segment needs recovery
Small-volume error, shared-path residue or insufficient mixing can change the actual ratio. Check results from the affected step before resuming the next action.
Retain current source, container identity, completed actions, valve or interface state and actual receiving information. Use the table to locate the stage that changed before deciding to retry, continue or restart. After recovery, recheck the relevant operating point before returning an uncertain action to the automatic cycle.