Locate the source, drive and receiving position for this task
Required inputs are solid-phase location and retention target, wash cycles, aspiration height, residual liquid, destination and downstream method requirements. Confirm liquid contact, pressure and destination along each illustrated section before defining drive and connection responsibilities.
Set height for wash cycles and solid-phase retention
Define the configuration for vessel geometry, wash cycles, peak inflow and the solid phase to retain. Confirm wash-head channel count and simultaneous-operation capability separately, and measure each well branch's resistance.
| Condition to compare | Information required | Selection affected |
|---|---|---|
| What must be retained | Whether magnetic beads, a solid phase or coating must be retained | Define permissible aspiration positions |
| Vessel interface | Cup bottom, well position, residual liquid and coverage | Coordinate washing and aspiration geometry |
| Concurrent inflow | Per-well supply, foam and peak waste load | Check total waste-removal capacity |
Compare product options against task requirements
The following candidate options match this page's responsibilities. Compare them against the operating conditions described, then check capacity, materials and interfaces on the product pages. Card images show representative configurations; suitability must still be confirmed at actual operating points and by application results.
| Product category | Corresponding role | Confirm before selection |
|---|---|---|
| Custom wash probe | Washing interface for probe interiors/exteriors or containers | Confirm contaminated surfaces, coverage, aspiration height and peak waste removal |
| DPL60 liquid diaphragm pump | Liquid supply or auxiliary circulation | Judge by peak demand and installed operating point, not free flow alone |
| DPGL800 gas–liquid diaphragm pump | Suitable gas–liquid aspiration or container-vacuum branches | Confirm inlet medium and waste architecture; not for continuous liquid-only transfer (100% liquid) |
Coordinate supply, separation and aspiration
Supply liquid, allow contact or mixing, separate and aspirate in sequence, with waste removal available throughout. Check wash-head misalignment, blocked ports and bottom clearance. Do not apply one aspiration height universally.
Action records should link commands, valve or interface states and actual received results. When raw material, container or sample identity changes, reconfirm shared paths and conditions permitting the next step. Completion of drive movement alone does not replace task acceptance.
Evaluate residue, losses and background by well
Measure residual liquid, well-to-well variation, solid-phase loss and method background across the whole plate or all cup positions. Accurate total supply can conceal inadequate local washing.
| Validation scenario | Measurement or observation | Interpretation and action |
|---|---|---|
| Residual liquid and solid phase | Measure wash residue and solid-phase loss together | Do not optimize solely for the driest possible vessel |
| Background and position-dependent variation | Compare washing results across vessels or wells | Locate unevenness in wash-head height, coverage and waste removal |
Record test medium, configuration, installed path and operating sequence; present routine operating points and boundary results separately. Calculations support design discussions, component documentation checks models, and installed measurements confirm the actual task.
After an abnormality or maintenance, first identify which segment needs recovery
Insufficient capture, incorrect aspiration height, uneven coverage or liquid accumulation can impair washing and recovery. Check actual results from the affected step before resuming the next action.
Retain current source, container identity, completed actions, valve or interface state and actual receiving information. Use the table to locate the stage that changed before deciding to retry, continue or restart. After recovery, recheck the relevant operating point before returning an uncertain action to the automatic cycle.