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Sampling Probes: Liquid Level, Bottom Clearance and Tip Delivery

Application guide

A sampling probe connects the source container, fluid path and receiver, affecting both aspiration and dispensing. Confirm immersion, wall contact, hanging droplets and clot risks using actual container geometry.

Application guides

Analytical instruments

Analytical instruments Overview
Liquid chromatography systems: locate the fluidic task before selecting pumps and valves
Spectroscopy and elemental analysis
Water analysis units
Analytical Sample Preparation
Analytical system integration
Piston Pumps in Analytical Instruments
Automated dilution with piston pumpsPiston Pump Titration
Syringe pump
Syringe-pump samplingMultiple dispensing with syringe pumpsSyringe-pump standard preparation and over-range sample dilutionSequential injection and flow analysis
Air-displacement pipetting pump
Disposable-Tip Sample TransferTip-based dilution and dispensingTip-based sample preparation
Valveless metering pumps
Repeated Dispensing with Valveless PumpsValveless-pump titrationCarrier-liquid and flow-cell supplyTwo-stream proportioning
Diaphragm pump
Wash-liquid supply and primingWorking-liquid replenishmentAuxiliary liquid circulationGas-liquid waste aspiration
Valves for Analytical Fluidics
MRV3 multi-source selectionHigh-pressure sample-loop injectionDetector Flow Diversion and BypassSolenoid-valve shutoff
Sampling, Washing and Mixing Components
Sampling needlesProbe-wash stationReaction-cup and microplate washingReaction mixing
Fluidic monitoring
Bubble and Liquid-State DetectionPressure and Blockage Monitoring
Tubing, fittings, filters and check valves
Tubing materials and dimensionsLow-retention connectionsFiltration and backflow protection

ON THIS PAGE

Locate the source, drive and receiving position for this taskDesign for minimum liquid level and bottom clearanceCompare product options against task requirementsCheck immersion, dispensing and internal and external probe washingMeasure low-volume delivery, retained droplets and carryoverAfter an abnormality or maintenance, first identify which segment needs recoveryContinue with tasks on the same branchReferencesRelated guides

01Locate the source, drive and receiving position for this task

Source-container liquid surface→Sampling-probe inlet→Metering drive and transfer→Target receiving location
Functional or operational sequence; confirm connections and branches for each state on the instrument

Required inputs are actual sample volume, remaining source volume, probe-tip height, aspiration/dispensing speed and system liquid. Confirm liquid contact, pressure and destination along each position in the diagram before assigning drive and connection responsibilities.

02Design for minimum liquid level and bottom clearance

Specify vessel dimensions, minimum liquid level, working volume, needle travel and acceptable remaining volume. Balance needle bore and path volume against flow, particle risk and sample budget.

Compare configuration conditions by actual task
Condition to compareInformation requiredSelection affected
Probe-tip positionImmersion depth, bottom clearance and low remaining volumePrevent aspiration of air or inlet blockage
Wetted surfacesProbe bore, external surface and sample propertiesCheck delivery and residue
Tip-end actionsWithdrawal, transfer, dispensing and suck-backEvaluate retained droplets and destination receipt

03Compare product options against task requirements

The following candidate options match this page's responsibilities. Compare them against the operating conditions described, then check capacity, materials and interfaces on the product pages. Card images show representative configurations; suitability must still be confirmed at actual operating points and by application results.

Move from task conditions to product configuration
Product categoryCorresponding roleConfirm before selection
Custom sampling probeSampling interface between source container and fluidic pathCompare custom geometry by minimum liquid level, probe-tip clearance and actual sample
EA standard piston pumpFinite-stroke meteringCompare configurations by routine dose, total cycle displacement and wetted materials
  • Custom sampling probe
  • EA standard piston pump

04Check immersion, dispensing and internal and external probe washing

Confirm liquid level and position before aspiration. Dispense using the specified receiving action, then wash internally and externally. Recheck bottom clearance after vessel changes or maintenance; correct mechanical position alone does not exclude air aspiration.

Action records should link commands, valve or interface states and actual received results. When raw material, container or sample identity changes, reconfirm shared paths and conditions permitting the next step. Completion of drive movement alone does not replace task acceptance.

05Measure low-volume delivery, retained droplets and carryover

Include normal and low-volume samples, measuring receipt, carryover and probe-tip droplets. Interpret blockage or leak alarms within the action window rather than monitoring motor position alone.

Link operating-point tests to interpretable results
Validation scenarioMeasurement or observationInterpretation and action
Minimum liquid levelCheck actual sampling and detection of air aspirationHandle affected actions according to sample identity
High/low sample switchingCheck volume and contamination recovery separatelyDistinguish internal probe residue from external droplets

Record test medium, configuration, installed path and operating sequence; present routine operating points and boundary results separately. Calculations support design discussions, component documentation checks models, and installed measurements confirm the actual task.

06After an abnormality or maintenance, first identify which segment needs recovery

Air aspiration, clots/particles, probe contact with a wall or leakage alter actual delivery. Stop the current action, confirm sample identity and resample or flag it according to the method.

Retain current source, container identity, completed actions, valve or interface state and actual receiving information. Use the table to locate the stage that changed before deciding to retry, continue or restart. After recovery, recheck the relevant operating point before returning an uncertain action to the automatic cycle.

  • Bring the fluidic diagram and operating-point records to discuss configuration

07Continue with tasks on the same branch

  • Sampling, washing and mixing components: define interfaces from vessel geometry
  • Probe Wash Stations: Identifying Contaminated Surfaces and Validating Blank Recovery
  • Cuvette and Microplate Washing: Residue, Solid Phase and Head Position
  • Reaction mixing: geometry, speed and reaction uniformity

References

View references (2)
  1. [1]Roche: cobas c 311 Operator's Manual — Sample Probe Wash and Clog Recovery
  2. [2]FOREACH: Sampling and Wash Probe Product Range

Related guides

  • Clinical chemistry sample transfer: from sampling architecture to probe-tip delivery
  • Probe Wash Stations: Identifying Contaminated Surfaces and Validating Blank Recovery
  • Pressure and blockage monitoring: establish action baselines before fault thresholds

Related Products

Custom sampling probe; representative configuration. Select capacity, materials and interfaces for the application.

Custom sampling probe

EA standard piston pump; representative configuration. Select capacity, materials and interfaces for the application.

EA standard piston pump

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Discuss your sampling-needle fluidics

Provide a fluid-path diagram, actual liquids and pressures in each state. Specify immersion, bottom clearance, low remaining volume, probe bore, external surface, sample properties, withdrawal, transfer, dispensing and suck-back. Compare configurations against the guide's validation objectives.

Contact an engineer