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Disposable-Tip Sample Transfer: Sealing and Receiving Actions

Application guide

Disposable tips help define consumable separation between samples, but transfer quality still depends on tip sealing, aspiration position and receiving actions. Changing consumables alone proves neither absence of contamination nor volume accuracy.

Application guides

Analytical instruments

Analytical instruments Overview
Liquid chromatography systems: locate the fluidic task before selecting pumps and valves
Spectroscopy and elemental analysis
Water analysis units
Analytical Sample Preparation
Analytical system integration
Piston Pumps in Analytical Instruments
Automated dilution with piston pumpsPiston Pump Titration
Syringe pump
Syringe-pump samplingMultiple dispensing with syringe pumpsSyringe-pump standard preparation and over-range sample dilutionSequential injection and flow analysis
Air-displacement pipetting pump
Disposable-Tip Sample TransferTip-based dilution and dispensingTip-based sample preparation
Valveless metering pumps
Repeated Dispensing with Valveless PumpsValveless-pump titrationCarrier-liquid and flow-cell supplyTwo-stream proportioning
Diaphragm pump
Wash-liquid supply and primingWorking-liquid replenishmentAuxiliary liquid circulationGas-liquid waste aspiration
Valves for Analytical Fluidics
MRV3 multi-source selectionHigh-pressure sample-loop injectionDetector Flow Diversion and BypassSolenoid-valve shutoff
Sampling, Washing and Mixing Components
Sampling needlesProbe-wash stationReaction-cup and microplate washingReaction mixing
Fluidic monitoring
Bubble and Liquid-State DetectionPressure and Blockage Monitoring
Tubing, fittings, filters and check valves
Tubing materials and dimensionsLow-retention connectionsFiltration and backflow protection

ON THIS PAGE

Locate the source, drive and receiving position for this taskSet immersion, speed and air gaps togetherCompare product options against task requirementsRecord tip pickup, sample identity and hanging dropletsEvaluate delivery and contamination using actual vesselsAfter an abnormality or maintenance, first identify which segment needs recoveryContinue with tasks on the same branchReferencesRelated guides

01Locate the source, drive and receiving position for this task

Source vessel→Immersed tip→Air displacement and motion→Target vessel
Functional or operational sequence; confirm connections and branches for each state on the instrument

Air displacement drives liquid within the tip, while motion brings the tip to the target location. The illustration shows the task sequence.

Define disposable-tip or fixed-probe architecture, sealing, working volume, liquid class, immersion position and dispensing action. Follow the diagram to confirm liquid contact, pressure and destination before assigning drive and connection roles.

02Set immersion, speed and air gaps together

Set immersion, speed and air gaps using working volume, minimum source volume and tip geometry. When retaining a suspension or product, avoid using a single water-based program that overlooks sample properties.

Compare configuration conditions by actual task
Condition to compareInformation requiredSelection affected
Aspiration positionImmersion depth, minimum liquid level and bottom clearancePrevent air aspiration or inlet occlusion
Motion conditionsDwell, withdrawal and motion between vesselsCheck retained droplets and dripping
Contamination-control strategyTip-change requirements and destination contact methodDefine sample identification and cross-contamination controls

03Compare product options against task requirements

The following candidate options match this page's responsibilities. Compare them against the operating conditions described, then check capacity, materials and interfaces on the product pages. Card images show representative configurations; suitability must still be confirmed at actual operating points and by application results.

Move from task conditions to product configuration
Product categoryCorresponding roleConfirm before selection
SMTP air-displacement pipetting pumpAir displacement with disposable tipsValidate tip sealing, liquid type and motion together
  • SMTP air-displacement pipetting pump

04Record tip pickup, sample identity and hanging droplets

Preserve source and target identities from tip pickup through disposal. Set post-aspiration waiting and dispensing position for the liquid class. If leakage, hanging droplets or air aspiration occurs, flag the current operation for review before proceeding.

Action records should link commands, valve or interface states and actual received results. When raw material, container or sample identity changes, reconfirm shared paths and conditions permitting the next step. Completion of drive movement alone does not replace task acceptance.

05Evaluate delivery and contamination using actual vessels

Measure actual received volume and run sample sequences with blanks to check cross-contamination. Recheck operating points after changing tip brand, motion or liquid formulation.

Link operating-point tests to interpretable results
Validation scenarioMeasurement or observationInterpretation and action
Vessel changesCompare deep and shallow vessels and low-volume deliveryCheck immersion and sealing before adjusting liquid-handling settings
Sample changeoverAssess blanks under the actual tip-change programValidate pipetted volume and external-surface contamination separately

Record test medium, configuration, installed path and operating sequence; present routine operating points and boundary results separately. Calculations support design discussions, component documentation checks models, and installed measurements confirm the actual task.

06After an abnormality or maintenance, first identify which segment needs recovery

Seal leaks, aspiration of air, hanging droplets or incorrect immersion can alter delivery. Check actual results from the affected step before resuming the next action.

Retain current source, container identity, completed actions, valve or interface state and actual receiving information. Use the table to locate the stage that changed before deciding to retry, continue or restart. After recovery, recheck the relevant operating point before returning an uncertain action to the automatic cycle.

  • Bring the fluidic diagram and operating-point records to discuss configuration

07Continue with tasks on the same branch

  • Air-displacement pipetting pumps: tips, liquid types and motion
  • Tip-Based Dilution and Dispensing: Linking Ratios to Actual Delivery
  • Tip-based preparation: supernatant removal, washing and eluate recovery

References

View references (2)
  1. [1]Hamilton: ZEUS Self-Contained Pipette Channel
  2. [2]FOREACH: SMTP2 / SMTP4 Pipetting Pump Datasheet

Related guides

  • Sampling Probes: Liquid Level, Bottom Clearance and Tip Delivery
  • Pressure and blockage monitoring: establish action baselines before fault thresholds
  • Tip-Based Dilution and Dispensing: Linking Ratios to Actual Delivery

Related Products

SMTP air-displacement pipetting pump; representative configuration. Select capacity, materials and interfaces for the application.

SMTP air-displacement pipetting pump

Related Technical Articles

What is a programmable automatic air-displacement pipetting pump?

A programmable automatic air-displacement pipetting pump is a liquid-handling component whose aspiration, dispense, speeds and sequence are set through control commands. This article focuses on air-displacement modules that use disposable tips in OEM instruments, with FOREACH SMTP2 as an example of operation, monitoring and selection limits.

2026-09-28View Details

What Is Pipetting? Manual and Automated Workflows Compared

Pipetting is the transfer of a specified volume of liquid from one location to another. Examples include moving 100 µL from a sample tube into a reaction vessel, or adding sample and diluent in a defined ratio.

2026-09-29View Details

Discuss your disposable-tip sample-transfer requirements

Provide a fluid diagram, the actual liquids and pressures in each state. Specify immersion depth, minimum liquid level and bottom clearance; holding, withdrawal and movement between vessels; and tip changes and target contact. Compare suitable configurations against the validation objectives described here.

Contact an engineer