Locate the source, drive and receiving position for this task
Define dose volume, dose count, first and last doses, retained volume and recovery after refill. Follow the diagram to confirm liquid contact, pressure and destination at each position before assigning drive and connection roles.
Include retained volume in the full-cycle capacity budget
Calculate usable capacity from dose volume, dispense count, retained volume and extra programmed displacement. Include replenishment in batch timing, and establish action resources separately for independent and shared channels.
| Condition to compare | Information required | Selection affected |
|---|---|---|
| Doses per cycle | Dose volume, channel count and retained volume | Calculate cycle capacity and refill points |
| Delivery at the outlet | Nozzle, retained droplets and receiving geometry | Define each dispensing action and end-of-cycle handling |
| Synchronization requirements | Channel paths and container positions | Distinguish synchronized drives from synchronized liquid delivery |
Compare product options against task requirements
The following candidate options match this page's responsibilities. Compare them against the operating conditions described, then check capacity, materials and interfaces on the product pages. Card images show representative configurations; suitability must still be confirmed at actual operating points and by application results.
| Product category | Corresponding role | Confirm before selection |
|---|---|---|
| HLD rotary-valve syringe pump | Aspiration, holding and dispensing with a rotary valve | Check source/outlet valve positions, capacity budget and replenishment timing |
| HMD solenoid-valve syringe pump | Metered aspiration and dispensing with solenoid valves | Check open/closed and power-off states, working volume and replenishment cycle |
Record dispensing by dose and well
After aspiration, dispense in well or container order, recording the current dose and remaining source volume. After refill, reconfirm the effective liquid column. Do not automatically restart an interrupted cycle from its beginning.
Action records should link commands, valve or interface states and actual received results. When raw material, container or sample identity changes, reconfirm shared paths and conditions permitting the next step. Completion of drive movement alone does not replace task acceptance.
Check first and last doses and channel differences
Check first, middle and last doses and each channel separately, covering simultaneous or sequential operation. Average volume can conceal an undersized final dose or local deviations, so retain individual-dose data.
| Validation scenario | Measurement or observation | Interpretation and action |
|---|---|---|
| First, middle and last doses | Measure actual doses individually | Investigate end-of-stroke capacity and liquid-column condition when trends change |
| Channel-to-channel variation | Compare at actual wells or vessels | Locate assembly, nozzle and calibration differences |
Record test medium, configuration, installed path and operating sequence; present routine operating points and boundary results separately. Calculations support design discussions, component documentation checks models, and installed measurements confirm the actual task.
After an abnormality or maintenance, first identify which segment needs recovery
Empty sources, bubbles, nozzle droplets and backpressure changes can affect first or last doses. Stop affected steps, validate recovery after priming and avoid duplicate dispensing.
Retain current source, container identity, completed actions, valve or interface state and actual receiving information. Use the table to locate the stage that changed before deciding to retry, continue or restart. After recovery, recheck the relevant operating point before returning an uncertain action to the automatic cycle.