Locate the source, drive and receiving position for this task
Liquid segments are aspirated into a holding coil first, then reversed or transferred through reaction and detection paths according to valve position. The illustration does not imply that all four sections remain in one-way series flow.
Define each liquid segment's volume and aspiration order, holding-coil capacity, transfer direction and detection window. Follow the diagram to confirm liquid contact, pressure and destination before assigning drive and connection roles.
Account for liquid segments, holding-coil volume and arrival time
Calculate each segment volume, holding-coil capacity, shared volume and detection delay. Path length and flow estimate transit time, but actual mixing and dispersion require response measurements. Set pressure limits for the actual method.
| Condition to compare | Information required | Selection affected |
|---|---|---|
| Liquid-segment budget | Segment volumes, aspiration order and holding-coil capacity | Define segment order and transfer direction |
| Arrival window | Path volume, installed flow and detection timing | Coordinate valve positions and data acquisition |
| Sample consumption | Priming, renewal and discard volumes | Distinguish total aspirated volume from effective analytical volume |
Compare product options against task requirements
The following candidate options match this page's responsibilities. Compare them against the operating conditions described, then check capacity, materials and interfaces on the product pages. Card images show representative configurations; suitability must still be confirmed at actual operating points and by application results.
| Product category | Corresponding role | Confirm before selection |
|---|---|---|
| HLD rotary-valve syringe pump | Aspiration, holding and dispensing with a rotary valve | Check source/outlet valve positions, capacity budget and replenishment timing |
| MRV3 multiport rotary valve | Multiple-source selection | Compare 10-, 16- and 24-port options and port diagrams; verify high-pressure suitability separately |
Align aspiration order with valve positions and measurement windows
Aspirate the specified liquid segments in sequence, reverse or transfer according to valve position, then align the measurement window with the arriving signal. Confirm holding-coil condition after sample changes and washing to prevent old segments entering the next cycle.
Action records should link commands, valve or interface states and actual received results. When raw material, container or sample identity changes, reconfirm shared paths and conditions permitting the next step. Completion of drive movement alone does not replace task acceptance.
Check peak response, bubbles and segment order separately
Compare arrival time, peak response, blank recovery and repeated cycles while measuring actual liquid volume. If response changes, check segment order, bubbles, valve timing and method-specific reaction conditions separately.
| Validation scenario | Measurement or observation | Interpretation and action |
|---|---|---|
| Segment order and response | Compare actual arrival order and detected peaks | When results change, check flow direction, air segments and shared volume |
| Recovery after sample changes | Cover blanks, standby and switching between liquids | Accept results only after confirming the valid analytical window |
Record test medium, configuration, installed path and operating sequence; present routine operating points and boundary results separately. Calculations support design discussions, component documentation checks models, and installed measurements confirm the actual task.
After an abnormality or maintenance, first identify which segment needs recovery
Incorrect segment order, dispersion, bubbles or detection timing can change analytical response. Check results from the affected step before resuming.
Retain current source, container identity, completed actions, valve or interface state and actual receiving information. Use the table to locate the stage that changed before deciding to retry, continue or restart. After recovery, recheck the relevant operating point before returning an uncertain action to the automatic cycle.