Locate the source, drive and receiving position for this task
Specify the retained phase or solid, waste and eluate destinations, aspiration height, allowable residual liquid and target. Trace contact surfaces, pressure and destinations along the diagram before assigning pump and connection roles.
Set aspiration height for retention or recovery objectives
Coordinate aspiration height and speed with solid-phase position and vessel geometry. Working-volume budgets should include residue and low-volume product recovery. Different preparation formulations may require different liquid-class programs.
| Condition to compare | Information required | Selection affected |
|---|---|---|
| What must be retained | Supernatant, precipitate, solid phase or eluate | Define retained material and waste for each step |
| Aspiration geometry | Interface, pellet position and allowable residual liquid | Set aspiration height and speed |
| Recovery path | Tip wetting, surface adsorption and transfer count | Control target loss and sample contamination |
Compare product options against task requirements
The following candidate options match this page's responsibilities. Compare them against the operating conditions described, then check capacity, materials and interfaces on the product pages. Card images show representative configurations; suitability must still be confirmed at actual operating points and by application results.
| Product category | Corresponding role | Confirm before selection |
|---|---|---|
| SMTP air-displacement pipetting pump | Air displacement with disposable tips | Validate tip sealing, liquid type and motion together |
Distinguish supernatant, wash-liquid and product destinations
After separation, remove supernatant, add wash liquid and perform processing and elution as required by the method, then transfer product to the correct destination. Organize tip changes and waste resources around contamination-control boundaries and platform scheduling.
Action records should link commands, valve or interface states and actual received results. When raw material, container or sample identity changes, reconfirm shared paths and conditions permitting the next step. Completion of drive movement alone does not replace task acceptance.
Evaluate residual liquid, retained solid phase and recovery together
Assess residual liquid, solid-phase loss, product recovery and blanks together. If volume passes but recovery falls, check aspiration position, materials and solid-phase condition rather than adjusting pump calibration alone.
| Validation scenario | Measurement or observation | Interpretation and action |
|---|---|---|
| Supernatant removal | Measure residual liquid and observe loss of solid phase or precipitate | Do not optimize solely for the driest possible aspiration |
| Eluate recovery | Compare actual collected volume and target response | Distinguish volume loss, adsorption and changes in the processing method |
Record test medium, configuration, installed path and operating sequence; present routine operating points and boundary results separately. Calculations support design discussions, component documentation checks models, and installed measurements confirm the actual task.
After an abnormality or maintenance, first identify which segment needs recovery
Incorrect aspiration position, phase carryover or surface adsorption may cause product loss. Check actual results from the affected step before resuming the next action.
Retain current source, container identity, completed actions, valve or interface state and actual receiving information. Use the table to locate the stage that changed before deciding to retry, continue or restart. After recovery, recheck the relevant operating point before returning an uncertain action to the automatic cycle.